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We added 20 ?L of 0.2?mol/L CaCl2 (Haemoscope Corporation) to a new preheated (37��C) reaction cup (TEG reaction cups and pins; Haemoscope Corporation), which had previously been placed in one of the TEG channels. The rested citrated blood tube was mixed gently and then 340 ?L of blood were added to each reaction cup. Special care was taken when the pipette was introduced in the cup, releasing the blood against the cup wall and avoiding air bubbles. All samples were handled and run by the same experienced operator (J.L.M). Tracings were obtained after 120?min of running time at 37��C. The following TEG parameters were measured and recorded: R time (R): time (min) from the addition of agonist (CaCl2) until the clot started forming, which evaluates the activity of plasma coagulation factors All [http://www.selleckchem.com/products/BIBF1120.html VEGFR inhibitor] data were analysed using a statistical software package (Prism version 4.0, GraphPad Inc., San Diego, CA, USA). Descriptive statistics were performed for all data analysed and normality was assessed using the D'Agostino & Pearson omnibus test. Intra-assay (between same sample), inter-individual (between animals) and intra-individual (at 12?h, 24?h, and 7 days after birth) variations were analysed in the healthy [http://en.wikipedia.org/wiki/Megestrol_acetate Megestrol Acetate] group using coefficients of variation (CV). Inter-individual variation was calculated from the first sample taken for each healthy foal. Intra-individual variation was evaluated for the five healthy foals to monitor the changes in coagulation over the first week of life. When data passed the normality test, the three groups were compared through one-way analysis of variance, followed by the Bonferroni post-test when differences were detected. If the data did not follow a Gaussian distribution, the groups were compared using the Kruskal-Wallis test, followed by Dunnet's post-test when required. Correlations between TEG variables and the haematological values and the conventional coagulation profiles from all samples were analysed using Pearson's test (when data followed Gaussian [http://www.selleckchem.com/products/umi-77.html UMI-77] distribution) or non-parametric Spearman test (when not normally distributed), and linear regression was performed for the variables when a statistically significant correlation was found. Statistical significance was set at P