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A part of the vastus lateralis was partially fixed in 10% neutral buffered formaldehyde, embedded in paraffin, and cut in 5 ?m sections. In haematoxylin-eosin (HE) stained sections, 400?��?10% myofibres of each animal were analysed. All fibres were counted as being centrally nucleated that contained at least one nucleus that was not associated with the sarcolemma. For the determination of the muscle fibres, CSA200?��?10% myofibres [http://www.selleckchem.com/products/abt-199.html Venetoclax] per animal were manually outlined using the analySIS? Image Processing software (Soft Imaging System GmbH, Muenster, Germany). Statistical analysis Statistical analysis was performed using SPSS 22.0 (IBM SPSS Statistics, Armonk, NY, USA). All data are given as mean �� standard deviation (SD). For all MRI data, results for the right and left [http://www.selleckchem.com/products/MDV3100.html Enzalutamide chemical structure] legs were averaged. Kolmogorov�CSmirnov test was used to test the normal distribution of data, and homogeneity of variances was confirmed by Levene's test. Depending on the results of these tests, either two-sided, unpaired t-testing, Welch test or the Mann�CWhitney U test were applied for analysis of the data. P?[http://en.wikipedia.org/wiki/Histone_demethylase Histone demethylase] receiving a control diet.24 Therefore, we raised the question of whether the same holds true for female Sprague�CDawley rats. Baseline (7 months of age) and subsequent (12 months and 16 months of age) MR examinations revealed no significant effect of the HFD [female HFD (fHFD] compared with the control group (fCD) (P?=?0.74; P?=?0.12; P?=?0.68, respectively). On the other hand, at 21 months of age, the CSA was even elevated in the fHFD group compared with the fCD (fCD: 1.12 cm2; fHFD: 1.20 cm2; P?