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Normal range for T3 was 0.5-1.9 ng/ml, for T4 was 4.8-11.6 pg/dl (females) and 4.4-10.8 pg/dl (males) and for TSH it was 0.3-6.3 �� IU/ml. Serum urea [http://www.selleckchem.com/products/VX-680(MK-0457).html VX-680 manufacturer] was measured using glutamate dehydrogenase-urease method in which urease hydrolyses urea to ammonia and ammonia formed further combines with alpha-ketoglutarate and NADH (reduced form of nicotinamide adenine dinucleotide [NAD]) to form glutamate and NAD, rate of oxidation of NADH to NAD is measured as a decrease in absorbance in a fixed time, which is proportional to urea concentration in the sample and optical density was taken at 340 nm. Serum creatinine was measured using Jaffe's method in which creatinine reacts with picric acid in alkaline medium to form an orange colored complex and rate of change of absorbance is measured at 505 nm using semi-auto analyzer. Serum cholesterol was measured using cholesterol oxidase (CHOD)- peroxidase (POD) method in which cholesterol ester in presence cholesterol esterase forms cholesterol, this free cholesterol in the presence of CHOD forms cholest-4-en-3-one and H2O2, this H2O2 in the presence of 4 aminoantipyrine and phenol forms quinone imine and absorbance of quinone [http://www.selleckchem.com/products/R406.html R406] imine so formed is directly proportional to cholesterol concentration and optical density was taken at 505 nm. Low density lipoprotein (LDL) cholesterol was measured using direct reagent kit in which LDL is measured in serum without the need for any offline pre-treatment or centrifugation, reagent consists of a reagent capable of solubilizing LDL specifically. Cholesterol esterase and chromogenic coupler react with this solubilize LDL to develop color, which is directly proportional to the concentration of LDL. High density lipoprotein (HDL) cholesterol was measured using Phosphotungstic method, in which chylomicrons, LDL and very low density lipoprotein (VLDL) are precipitated from serum by phosphotungstate in the presence of divalent cation such as magnesium, the HDL cholesterol remains unaffected, which is later [http://en.wikipedia.org/wiki/Peptide_bond Peptide bond] on estimated CHOD-POD method. Results were expressed as mg/dl. Normal range for urea was 15-45 mg/dl, for creatinine was 0.5-1.5 mg/dl for cholesterol was 150-250 mg/dl, for TG was 35-165 mg/dl, for LDL was