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08��1.53 and 13.53��1.02 cmH2O, respectively, and significantly lower compared with the BOO group (P[http://www.selleckchem.com/products/s-gsk1349572.html check details] muscle layer. In the control group, it was observed mainly in the luminal surface of the urothelium. In the BOO group, the expression of ��3 nAChR was significantly increased in the urothelium compared with the control group. However, there was no significant difference from the control group in the detrusor muscle layer (Fig. 2). The ��7 nAChR expression was observed in both the urothelium and detrusor muscle, unlike ��3 nAChR. In the control group, ��7 nAChR expression [http://www.selleckchem.com/products/Dasatinib.html selleck kinase inhibitor] was observed in all layers of the urothelium. In the BOO group, ��7 nAChR expression further increased in the urothelium and detrusor muscle compared with the control group (Fig. 3). Fig. 2. Immunofluorescence staining of ��3 nicotinic acetylcholine receptor of urothelium in the control and BOO groups. (A) Control urothelium, (B) BOO urothelium, (C) control muscle, and (D) BOO muscle. BOO, bladder outlet obstruction. Fig. 3. Immunofluorescence staining of ��7 nicotinic acetylcholine receptor of urothelium in the control and BOO groups. (A) Control urothelium, (B) BOO urothelium, (C) control muscle, and (D) BOO muscle. BOO, bladder outlet obstruction. Changes in the Expression of ��3 and ��7 nAChRs by Western Blot Analysis Expressions of ��3 nAChR and ��7 nAChR were confirmed in the mucosa and detrusor muscle. Alpha3 nAChR expression was significantly increased in the mucosa of the BOO group compared with the control group (P muscle were [http://en.wikipedia.org/wiki/SAR1B SAR1B] similar for the BOO and control group. Expression of ��7 nAChR in the BOO group significantly increased both in the mucosa and detrusor muscle compared with the control group (P