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<p>Page créée avec « Footnotes 1http://imagej.nih.gov/ij/ 2http://www.gelanalyzer.com Supplementary Material The Supplementary Material for this article can be found online at: http://journal.... »</p> <p><b>Nouvelle page</b></p><div>Footnotes 1http://imagej.nih.gov/ij/ 2http://www.gelanalyzer.com Supplementary Material The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fnsyn.2016.00013/abstract Supplementary Figure 1 Characterization of a novel polyclonal anti-SPAR antibody. (A) Specificity testing of the anti-SPAR antibody. HEK293T cells were co-transfected with GFP-SPAR and either the control vector (Vector) or SPAR RNAi (for characterization of [http://www.selleckchem.com/products/Temsirolimus.html http://www.selleckchem.com/products/Temsirolimus.html] these constructs see Richter et al., 2007). Western blotting of the corresponding cell lysates with anti-SPAR (left panel) and anti-GFP (right panel) showed reduction of GFP-SPAR in the presence of SPAR RNAi. (B) HEK293T cells were further transfected with GFP-SPAR, GFP-SPAR2 or GFP-SPAR3. Western blotting of the corresponding cell lysates with anti-SPAR or anti-GFP showed that this antibody is not cross-reactive to SPAR2 and SPAR3. Click here for additional data file.(158K, jpeg) Supplementary Figure 2 Analysis of PSD95 and LAPSER1 after ProSAPiP1 overexpression and knockdown in [http://en.wikipedia.org/wiki/Hsp90 HSP90] mature primary hippocampal neurons. (A) Primary hippocampal cultures were infected with either FUGW empty vector (Vector) or GFP-ProSAPiP1 and stained for PSD95 (red) or LAPSER1 (red) on DIV28 as indicated. The intensity of LAPSER1-positive puncta was significantly increased. (B) Primary hippocampal cultures were infected with either Scr or RNAi and stained for PSD95 (red) or LAPSER1 (red) [http://www.selleckchem.com/products/Etopophos.html Etoposide price] on DIV28 as indicated. No significant difference was observed. (A,B) Scale bar: 10 ��m. Statistical analysis was performed using unpaired two-sided t-test. *p</div>

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