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, [23]. The total antioxidant capacity of plasma was evaluated by applying the FRAP assay (ferric reducing antioxidant power or ferric reducing ability of plasma) according to the method of Benzie & Strain [24]. Reduced glutathione in [http://en.wikipedia.org/wiki/ATPase ATPase] blood was estimated by the method of Beutler et al., [25]. Reactive oxygen species can damage the DNA. DNA damage can be determined by the method of Lee et al., [26] with slight modifications. Genomic DNA was isolated by salting out method [27]. DNA was incubated with Fenton��s reagent containing test sample and the final volume of the mixture was raised up to 20��l. The mixture was then incubated for 30min at 37��C and the DNA was analysed on a 1% agarose gel electrophoresis followed by ethidium bromide staining. Finally gel was analysed using uvitec gel document (Cambridge, UK). Statistical Analysis The data are expressed as the mean and standard deviation of three parallel measurements. The data were subjected to one-way-analysis of variance (ANOVA). Duncan��s test was used to determine significant differences. p-values �� 0.05 were considered as level of significance. The statistical analysis was carried [http://www.selleckchem.com/products/vinorelbine-tartrate.html Vinorelbine] out using SPSS for Windows software (Version 13.0, SPSS, Chicago, IL, USA). Results The effect of curcumin on ischemia modified albumin formation is presented in [Table/Fig-1,?,2].2]. We have noted that, when serum was exposed to hydroxyl radicals the production of ischemia modified albumin was highly significant (p[http://www.selleckchem.com/histone-demethylases.html Histone Demethylase inhibitor manufacturer] antioxidant capacity of plasma compared with the control (p

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Notekey15 : Page créée avec « , [23]. The total antioxidant capacity of plasma was evaluated by applying the FRAP assay (ferric reducing antioxidant power or ferric reducing ability of plasma) accordin... »