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It has been suggested that LPS is a TLR4 ligand and stimulates mast cells in a TLR4-dependent manner to produce a series of cytokines and chemokines (McCurdy et al. 2001; Supajatura et al. [http://www.selleckchem.com/products/lee011.html LEE011] 2002; Qiao et al. 2006). We therefore investigated whether TLR4 is the molecule responsible for the effects of LPS on mast cells. The results show that LPS increased TLR4 mRNA levels in a time-dependent manner (Fig. 4A). Blocking TLR4 with its specific inhibitor, Cli-095 (1 ��g ml?1, applied 6 h before addition of LPS), significantly diminished the LPS enhancement of Fc?RI-mediated degranulation and Ca2+ mobilization (Fig. 4B and C), as well as TG-elicited Ca2+ entry (Fig. 4D). Concomitantly, Cli-095 decreased LPS-mediated upregulation of Orai1 and STIM1 mRNA and protein levels [http://www.selleckchem.com/products/mi-773-sar405838.html SAR405838] (Fig. 4E�CH). These results collectively indicate that TLR4 is an important molecule for LPS regulation of Ca2+ mobilization and degranulation in mast cells. Lipopolysaccharide enhanced AHR The 24 h OVA challenge remarkably increased AHR. The average Penh values at 30, 50 and 100 mg ml?1 methacholine in OVA-treated mice were significantly higher than that in PBS-treated control mice (P [http://en.wikipedia.org/wiki/Resiquimod Resiquimod] a significant increase of histamine level in both serum and BALF, indicating a burst of mast cell degranulation (Fig. 6A and B). Although LPS alone had no effect on histamine release, it synergistically increased histamine release with OVA challenge. Histamine concentrations in both serum and BALF in LPS-treated allergic mice were significantly higher than those in LPS-untreated allergic mice (P

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Scenecold6 : Page créée avec « It has been suggested that LPS is a TLR4 ligand and stimulates mast cells in a TLR4-dependent manner to produce a series of cytokines and chemokines (McCurdy et al. 2001; ... »