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Figure 3. Phosphorylation of Akt, AS160, and TBC1D1 in rat soleus muscles 2 h after treadmill exercise (180 min at 9 m/min). See the Materials and Methods section for details. Frozen muscles were used to measure the phosphorylation of Akt Ser473 (A), Akt Thr308 ... When muscles were dissected out 2 h postexercise, there was no exercise effect on the insulin�\independent basal phosphorylation of either AS160 Thr642 or AS160 Ser588 (Fig. ?(Fig.3C3C and D). AS160 Thr642 phosphorylation was increased with submaximal Docetaxel concentration (50 ��U/mL) insulin treatment in the muscles of both nonexercised and exercised rats (Fig. ?(Fig.3C).3C). On the other hand, the AS160 Ser588 phosphorylation was not significantly increased with submaximal (50 ��U/mL) insulin treatment in the muscles of the nonexercised rats but it was significantly increased in those of the exercised rats (P 100 �� 4 AU, n = 8; exercise: 102 �� 6 AU, n = 8). When we calculated Vatalanib (PTK787) 2HCl the submaximal (50 ��U/mL) insulin�\stimulated increase above the basal level of AS160 phosphorylation by subtracting the basal value from the insulin�\stimulated value, calculated ? insulin for phosphorylation of AS160 Thr642 in the exercised muscles was not significantly greater compared to the values of the nonexercised muscles (nonexercise: 182 �� 21 AU, n = 8; exercise: 200 �� 20 AU, n = 8). Calculated ? insulin for phosphorylation of AS160 Ser588 in the exercised muscles was also not significantly EX 527 price greater compared to the values of the nonexercised muscles (nonexercise: 98 �� 24 AU, n = 7; exercise: 186 �� 45 AU, n = 7). When muscles were dissected out at 2 h postexercise, there was no significant exercise effect on the insulin�\independent basal phosphorylation of TBC1D1 Thr590 (Fig. ?(Fig.3E).3E). The insulin�\independent basal phosphorylation of TBC1D1 Ser237 was 30% lower in the exercised muscles compared to the nonexercised muscle (P