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According to DNA cytometry (Figure 3), at the 11th day of gestation, the proliferative subpopulation contains not only diploid (34.3%), but tetraploid (44.8%) and 8c cells (16.1%), most probably due to polyploid mitoses (Zybina et al., 2005). Non-proliferative ��border�� subpopulation has in general a higher ploidy level: 2c cells are very rare (1.6%) and the vast majority constitute 4c (53.1%) and 8c (34.7%) cells, probably due to the preferential invasion of cells of higher ploidy. At the 14th day of gestation, ploidy of the proliferative subpopulation does this website not change significantly (Figure 3). The two mitotically inactive subpopulations of ��border�� and spindle-like cells reach higher ploidy levels. Thus, the ��border�� cells are almost devoid of diploid cells (0.8%), whereas 50.4% of them are 4c and 46.3% 8c; there are a few 16c cells tuclazepam (2.5%). In the spindle-like subpopulation the ploidy is a slightly lower compared to the ��border�� ones. Thus, the share of diploid cells falls to 5.3% whereas 4c cells constituted 57.0%, and 8c 35.8%; there was also a small amount of 16c cells (1.1%) and 32c (1.1%). The difference in percentage of various ploidy classes between different cell subpopulations is statistically significant (��2-test giving P?Tofacitinib datasheet prior to 12.5 days of gestation, cells that had invaded endometrium beyond the main giant cell layer are tightly located around arteries, but show no general invasion of the interstitium in the decidua and venous sinuses (Adamson et al., 2002). Glycogen trophoblast cells invade the decidua outside the trophoblast giant cell layer after only 12.5 days of gestation, the invasion having a diffuse interstitial invasive pattern (Adamson et al., 2002). Such an invasion of murine trophoblast cells has been confirmed by the expression of MMP9 beginning only from the 13.5 day of gestation (Coan et al.