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A pyelotomy or ureterotomy incision was then made with a blade. This incision was extended to a length sufficient for stones to be picked up by a laparoscopic grasper (Fig. 2A). An additional lithoclast was used in one case to fragment a relatively large stone that could not be retracted. After the main stone was removed, flexible nephroscopy was used with a trocar and pyelotomy to look for and pick up stones scattered in multiple calyces if these were suspected on the basis of the preoperative imaging study (Fig. 2B). If a residual stone was detected, a stone basket was used via flexible nephroscopy. Stones were removed by using a specimen retrieval bag and were extracted from the learn more body through the 12-mm working port. After removal of the stone fragments, a decision was made to place a ureteral stent. The incision site was repaired by use of 4-0 absorbable sutures. A drain was placed in all patients. The Foley catheter, surgical drain, and ureteral stent were removed 1 to 3 days, 2 to 4 days, and 2 to 4 weeks after surgery, respectively. FIG. 1 Placement of ports (A, B) for laparoscopic stone surgery in the right (C) and left (D) kidneys. FIG. 2 Stone removal using laparoscopic graspers or right-angled clamps via an incision site (A) and the use of flexible nephroscopy to pick up residual stones (B). 3. Statistical analysis Data were expressed as means��standard Edoxaban deviations. Surgical outcomes according to the use of flexible nephroscopy were analyzed by use of chi-square tests. Logistic regression analysis was performed to find the clinically significant predictors in determining Brefeldin A ic50 operative success. Values were considered to be statistically significant when p