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Total RNA from frozen tissues had been extracted with Trizol Reagent (Invitrogen, Gaithersburg, MD, United states) adhering to the manufacturer's recommendations
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Total RNA was reverse transcribed with oligo primer using the M-MLV reverse transcriptase for RTPCR
Total RNA was reverse transcribed with oligo primer working with the M-MLV reverse transcriptase for RTPCR
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Treated mice had been examined twice per week for detecting the presence of skin papillomas, which were not scored as constructive till they reached a minimum of 1 mm in diameter
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