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63%, 72.47%, 56.39%, while the scramble transfected negative control showed a cell viability of 96.51%, 86.51%, and 89.78% at the end of 24, 48, and 72 hours of incubation, respectively, compared to untransfected control cells. Increase of apoptosis in mimic-transfected RB cells In Y79 cells, the annexin assay revealed that treatment with miR-486-3p and miR-532-5p mimics resulted in the induction of late apoptotic cells to about 30.9% and 30.6%, while the scramble mimic treatment resulted in 2.62% compared to the untransfected cell control, at the end of 48 hours of incubation. Similarly, treatment with miR-486-3p and miR-532-5p mimics in Weri Rb1 cells resulted in the induction of late apop-totic cells to about 19.6% and 21.8%, while selleck the scramble mimic treatment resulted in 13.5% compared to the untransfected control cells at the end of 48 hours of incubation. A significant change GSK1349572 price (P-value SAR1B resulted in the alteration of G2/S phase cells to ?53.1% and 54.9%, respectively, while treatment with the scramble mimics resulted in 47.9% at the end of 48 hours of incubation. Though a significant change of G2 /S phase cell percentage was observed in Y79 cells, no significant change was observed in Weri Rb1 cells. Discussion The standard methodology to perform functional analysis of MMIs would require array-based mRNA and miRNA global expressions or RNA sequencing inputs. These approaches would require large investment, including a huge quantity of purified RNA and the economic resources to perform two different arrays, which in turn limits number of paired miRNA�CmRNA datasets in the available public repositories.29 Thus data mining from these repositories would provide immediate information about the paired miRNA�CmRNA networks and their gene target regulations. In this study, we used an in silico approach to identify miRNAs regulating the panel of genes [categorized as Group I genes (HMGA2, MYCN, SYK, FASN), Group II genes (TACSTD1, ABCG2, CD133, CD44, CD24), and Group III genes (p53 and MDM2) involved in RB tumoriogenesis; Figure 1]. With the removal of redundancy, 1948 common miRNAs were obtained from this miRNA approach.