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3a - 3c Histograms showing cell viability at a) day 7: Cyclin-dependent kinase 3 significantly lower in the TA groups than in the control group. Also, cell viability in the high TA group was significantly lower than that in the low TA group. b) Day 14: cell viability in the low TA group ... Apoptotic cell analysis The apoptotic cells were stained green during immunofluorescence staining (Fig. 4). In the control group, few apoptotic cells were seen at days 7, 14 and 21. In the low TA group, a higher number of apoptotic cells was observed at day 7 compared with that in the control group. The appearance of apoptotic cells decreased at days 14 and 21 compared with that at day 7. In the high TA group, numerous apoptotic cells were observed at day 7. At days 14 and 21, the number of apoptotic cells did not decrease. In the low TA group, the rate of apoptotic cells was significantly reduced at days 14 and 21 compared with that at day 7 (day 7: 25.5%, sd 9.3; day 14: 16.8%, sd 5.8 and day 21: 11.6%, sd 5.8). However, in the high TA group, the rate of apoptotic cells was not reduced at any point (day 7: 37.5, sd 12.2%; day 14: 39.9%, sd 18.6 and day 21: 37.5%, sd 14.6). Fig. 4 Immunofluorescence staining showing apoptotic cells (green). In the control, there were few apoptotic cells. Apoptosis in the low TA group decreased over time. The expression of apoptosis was increased in the high TA group, and did not change. The table ... Quantitative real-time polymerase chain reaction Quantitative real-time PCR revealed that the mRNA Selleckchem Epigenetics Compound Library expressions of caspase-3, 7, 8 and 9 in the TA-treated group were significantly increased at day 7 compared with those in the control group (p AR42 revealed that the mRNA expressions of caspase-3, 7, 8, and 9 in the TA-treated group were significantly increased at day 7 compared with a) those in the control group (p?