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The specific changes in the brain regions were analysed using a multiple comparisons procedure (Sidak's) where an overall difference between the groups was identified. The difference of dopamine ratios between the CML and control supplemented groups was analysed using unpaired t-tests. The correlation between synaptophysin staining and our previously published MWM JAK inhibitor data (5) was also analysed using Pearson tests. The data are presented as mean��standard error of the mean (SEM). A p value of less than 0.05 was considered to be significant. Results Synaptophysin Synaptophysin staining was densely distributed within different sub-regions of the hippocampus, particularly in the CA3 (Fig. 1A) and CA4 (Fig. 1B) sub-regions. The microscopy images were representative of synaptophysin staining in the mossy fibres of the CA3 sub-region, and the CA4 sub-region, where it was defined in the area between the dorsal and ventral horns of the dentate gyrus. We evaluated both the average area (Fig. 1C) Quinapyramine and the average density (Fig. 1D) of the staining in the sub-regions of the hippocampus. Fig. 1 Changes in synaptophysin in the CA3 and CA4 sub-regions of the hippocampus. Representative images showing synaptophysin staining in the CA3 (A) and CA4 (B) hippocampal regions. The average area (C) and the average density (D) of synaptophysin staining ... Two-way ANOVA showed that the average area of staining was significantly different between the two regions (F[2,81]=84.55, pNeratinib in vivo groups (F[1,81]=7.95, p=0.0076), with a moderate interaction between the brain regions and the treatment groups (F[2,81]=4.46, p=0.014, Fig. 1C). Post-hoc tests showed that the average area of synaptophysin staining was significantly larger in the CA3 sub-region of CML group (p