Concepts, Methods Combined with Techniques For the MASP1

05) ( Fig.?2B). The effect of drugs in parasite replication was also evaluated by T.?gondii growth inhibition as shown in Table?1. The treatment with increasing concentrations of azithromycin resulted in significant inhibition of tachyzoite growth from 13 to 41% (P?AZD6738 Photomicrographs of BeWo cells showed a greater number of T.?gondii infected cells in those that were not treated ( Fig.?3A) as compared to those treated with azithromycin ( Fig.?3B,C) or spiramycin ( Fig.?3D). Non-infected BeWo cells showed significant increase in MIF production after treatment with azithromycin (100 and 200?��g/mL) and with this website any concentration of spiramycin as compared with non-treated control (Fig.?4A). Comparison of the treatments showed higher MIF production for spiramycin than azitromycin only in the highest concentration (400?��g/mL) (P?MASP1 control cells ( Fig.?5A,C). In contrast, treatment of infected cells with azithromycin (100 and 200?��g/mL) or spiramycin (400?��g/mL) slightly increased TNF-�� production compared with non-treated infected cells (P?