Frustrated With 17-AAG ? Then Simply Check This Out !!

1-2.0 mg/ml) in methanol were prepared. To 0.5 ml of each solution, 0.1 ml, 1 mM FeCl2 was added and mixture was allowed to stand for 5 min. The reaction was initiated by the addition of 0.2 ml of 5 mM ferrozine, the 17-AAG nmr mixture was made up 4 ml with ethanol and shaken vigorously and left standing at room temperature for 10 min. Absorbance of the solution was then measured spectrophotometrically at 562 nm. Increase in absorbance, indicates poor Fe2+ chelating activity. Ethylene diamine tetra-acetic acid (EDTA) was used as positive control. Blank containing no extract was used as the negative control. The percentage inhibition of ferrozine- Fe2+ complex formation was calculated as: [(A0- As)/A0] ��100. Where: A0= Absorbance of the control/blank; As = the absorbance of the extract learn more or EDTA (positive control). Statistical analysis All data were expressed as mean �� standard deviation of mean. Analysis of variance was performed by ANOVA procedures and P such as saponins, phenolic compounds and steroidal nucleus while anthraquinones and alkaloids were not detected. Acute toxicity study In this study, oral administration of graded doses of the extract to the mice did not produce any significant changes in behavior, breathing, cutaneous effects, sensory nervous system responses or gastrointestinal effects. No mortality or any toxic reaction like convulsion, ataxia, diarrhea or increased dieresis was recorded in any group after observation for 72 h after administering the extract to the mice. The extract was safe at the tested doses. Analgesic activity Effect of extract on acetic acid-induced writhing The results of the acetic acid writhing test in mice are shown in Table 1. At all tested doses, the extract inhibited the writhing responses of the mice caused by the intra-peritoneal administration of acetic acid in dose dependent fashion. The percentage inhibition of the writhing reflex also increased from zero in the negative tiospirone control group to 70.53% in the highest dose of the extract (1000 mg/kg) treated group which was higher than the 62.63% observed in the group that received acetyl salicylic acid (100 mg/kg). The inhibitory effects of aqueous, ethyl acetate, chloroform and hexane fractions (200 mg/kg) were 77.52, 72.93, 3.87 and 41.86%, respectively. Table 1 Effect of methanol extract of Petersianthus macrocarpus stem bark on acetic acid induced writhing in mice Effect of extract in hot-plate test The results of analgesic activity of the extract assessed using hot plate test are shown in Table 2. The extract at 200 and 500 mg/kg produced slight increase in mean post reaction time when compared to the group that received 7% DMSO (negative control group). However at 1000 mg/kg, there was a significant (P