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25% NaClO?+?0.9% normal saline?+?distilled water (Er:YAG/NaClO/NS/DW), Er:YAG?+?0.9% normal saline?+?distilled water (Er:YAG/NS/DW), Er,Cr:YSGG, and aPDT) and two control groups (5.25% NaClO as positive control and 0.9% normal saline (NS) as negative control). SCR7 mouse The numbers of bacteria on the surface of root canal walls and at different depths inside dentinal tubules before and after treatment were analyzed by means of one-way analysis of variance (one-way ANOVA). The morphology of bacterial cells before and after treatment was examined by scanning electron microscopy (SEM). After treatment, the bacterial reductions in the experimental groups and the positive control group were significantly greater than that of the negative control group (P?Mianserin HCl canals. Er:YAG/NaClO/NS/DW seems to be an ideal protocol for root canal disinfection during endodontic therapy. Lasers Surg. Med. 44: 824�C831, 2012. ? 2012 Wiley Periodicals, Inc. ""2961" "Background? Inclusion of sexual activity in the macaque model for topical microbicide safety evaluation would more closely mimic human use of topical microbicides and provide a more rigorous safety assessment. Methods? Initially, male�Cfemale partners were monitored in cohousing arrangements to determine whether macaques would copulate ad libitum. The logistics of performing vaginal examinations before and after coital visits were analyzed and optimized. Findings from cervicovaginal examinations conducted before and after sexual activity were compared. Results? Coital activity was reliably observed in the majority of cohousing sessions, representing all phases of the menstrual cycle. Female macaques were trained to be restrained while fully alert for pre-coital vaginal sampling. Post-coital examinations occur under general sedation. Post-coital examinations reveal alterations to tissues, microbiology, and pH compared with pre-coital visits. Conclusions? This work clearly Entinostat solubility dmso demonstrates that it is feasible to incorporate sexual activity in the macaque model for topical microbicide safety assessment. ""2962" "Background? Animals undergoing experimental manipulations, such as exposure to radiation, may exhibit physiologic and behavioral signs of pain and distress. Telemetry permits close monitoring of these parameters for early and effective management during procedures. Methods? Radiotelemetric units were surgically implanted into 24 Macaca mulatta before 6.5-Gy cobalt-60 ��-photon irradiation. Each unit transmitted electrocardiogram, intrathoracic pressure, and body temperature leads.