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The gaze behavior toward each facial expression was calculated for each child by dividing the fixation duration on the face AOI for each expression (e.g., surprised) by the total fixation duration on the face AOI for all expressions (surprised, happy, neutral, angry, and sad). 3. Results 3.1. Total Time Spent Looking at the Face The mean proportion of the total time spent looking at the face AOI was 65.1% (SD = 18.7) for children with ASD and 77.5% (SD = 20.0) for TDs. The diagnosis �� facial expression ANOVA on % Face revealed no effects for diagnosis, F(1, 24) = 2.49, p = 0.128, ��p2 = 0.09, facial expression, F(4, 96) = 1.05, p = 0.384, ��p2 = 0.04, or diagnosis �� facial expression interaction, F(4, 96) = 0.31, p = 0.869, ��p2 = 0.01. 3.2. Eyes and Mouth Ratios check details Figure 2 shows the mean % Eyes and % Mouth for each facial expression category. Figure 2 Mean proportion of total time spent looking at the eyes AOI and the mouth AOI for each facial expression category. The error bars indicate BML-190 95% confidence intervals. The diagnosis �� facial expression ANOVA on % Eyes indicated a significant effect for facial expression, F(4, 96) = 5.01, p = 0.001, ��p2 = 0.17, but no effect for diagnosis, F(1, 24) = 0.80, p = 0.380, ��p2 = 0.03, or diagnosis �� facial expression interaction, F(4, 96) = 0.27, p = 0.895, ��p2 = 0.01. Post hoc t-tests for the main facial expression effects revealed significantly shorter fixation durations on the eyes for the surprised expression compared to the sad expression, t(25) = ?3.61, p = 0.001, and the angry expression, t(25) = ?3.31, p = 0.003. Both groups tended to look for a shorter time at the eyes for the happy expression compared to the sad expression, t(25) = ?2.83, p = 0.009 (n.s. with Bonferroni correction). The diagnosis �� facial expression ANOVA for % Mouth indicated a significant effect for facial expression, F(4, 96) = 8.54, p http://www.selleckchem.com/products/Cyclopamine.html = 0.02. Post hoc t-tests for the main facial expression effects revealed significantly longer fixation durations on the mouth for the surprised expression compared to the sad, t(25) = 4.47, p