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Tissue specimens were fixed in 10% buffered formalin and embedded in paraffin wax using standard procedures. A blood sample (100??L) was obtained from the inferior vena cava EX 527 order and stored on filter paper. Four-micrometre sections were stained with haematoxylin & eosin. Detection of integrated HIV provirus was determined by qualitative DNA PCR using the standard Amplicor HIV-1 kit (Roche, Johannesburg, South Africa) on blood collected on filter paper. In all cases the presence of PfIE was investigated in CNS, liver, lung, spleen, kidney, heart, pancreas, uterus and placenta, by scanning all slides at low magnification (100��) with polarized light. In at least one of the slides of each organ, 50 high-power fields (1000��) were analysed to completely exclude the presence of PfIE. We selected as study group all cases showing unequivocally the presence Selleckchem Docetaxel of any PfIE in any of the organs of the autopsy. The medical records of all patients included in the study were reviewed to retrieve the clinical and epidemiological data. Under immersion oil (1000��) 100 perpendicularly cross-sectioned capillaries were evaluated and the contents were counted by a single observer in a section of the following organs: CNS, lung, kidney, heart, pancreas and uterus. A capillary was defined as a blood vessel, circular or oval in profile, with a maximum to minimum diameter of Vatalanib (PTK787) 2HCl structure. To provide accurate p-values, 5000 simulations were performed to approximate the distribution of Skillings�CMack values under the null hypothesis, taking into account the particular missing-data structure and tied rankings. The level of significance was set at p?