Hot Hints Into Gemcitabine Never Before Exposed

Figure 4 Effect of the different concentrations of the recombinant proteins on nitric oxide (NO) generation. Cells without any treatment were used as a control. Data are presented as mean��SD of 3 independent experiments. *Indicates a significant (PSuccimer 5 Recombinant ESAT-6, CFP-10, and ESAT-6/CFP-10 proteins antagonized lipopolysaccharide (LPS)-induced nitric oxide (NO) production. Differentiated THP-1 cells without any treatment were used as a control. Data are presented as mean��SD of 3 independent ... Effect of Recombinant Proteins on iNOS Enzyme Activity in Macrophages Table 1 demonstrates the effects of the recombinant ESAT-6, CFP-10, and ESAT-6/CFP-10 selleck inhibitor proteins on iNOS enzyme activity in the differentiated THP-1 cells. The treatment of the differentiated THP-1 cells with LPS (5 ?g/mL) significantly increased iNOS enzyme activity over 24 hours (Pcheck details NO by iNOS.23 LPS-induced oxidative burst in macrophages assists in killing invading pathogens.24,25 Macrophages respond to LPS by phosphorylating many intracellular signaling molecules.24,26,27 This initiates a cascade of signaling events, leading to the secretion of inflammatory cytokines such as IL-1, IL-6, tumor necrosis factor alpha (TNF-a), and antimicrobial immune responses.28 Trajkovic et al.29 (2002) showed that CFP-10 was able to bind to the surface of mouse J774 macrophage-like cells and stimulate the secretion of the proinflammatory cytokine TNF-a, but it markedly reduced NO release on subsequent stimulation with LPS or Mtb cell lysate.