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Fig. 1C shows results for day 14 samples. A pattern similar to day 7 was observed for day 14 samples. Untreated sample (lane 1) and BCT reagent-treated learn more sample (lane 2) were PCR amplified and DNA bands appeared to be of similar intensities. No bands were observed for formaldehyde treated (lane 3) and glutaraldehyde treated (lane 4) samples. DNA samples were prepared and treated with fixative reagents as described in the ��Materials and methods�� section. All samples were incubated at 22?��C and an aliquot of DNA was removed from each sample on days 0, 7 and 14 for qPCR. Fig. 2 demonstrates the effect of the three fixative reagents on DNA concentration, as determined by qPCR. Untreated control DNA samples showed a slight but statistically significant decrease in DNA concentration only at day 7. DNA concentration in DNA samples treated with BCT reagent did not change over the 14 day time period. However, statistically significant decreases in DNA concentration were observed in DNA samples treated with formaldehyde and glutaraldehyde over a 14 day period. DNA fragment amplified from GAPDH gene, was spiked into blood samples as described in the ��Materials and methods�� section. Blood was treated with fixative reagents as described. CGK 733 Aliquots of blood were removed from each tube at indicated time points, and plasma DNA was extracted and quantified by qPCR. Fig. 3 shows the effects of fixative reagents on plasma DNA concentration. Plasma DNA concentration in untreated control and BCT reagent-treated blood samples did not changed during 14 days of incubation at 22?��C. Plasma DNA concentration in blood samples treated with formaldehyde and glutaraldehyde showed a gradual, statistically significant decrease over the 14 days of incubation at 22?��C. To study the effect of fixative reagents on double-stranded DNA structure, DNA was exposed to fixatives for various time periods. Fig. 4 shows the effect of the fixative reagents on DNA. Untreated DNA and BCT reagent-treated DNA, showed no statistically significant Trichostatin A manufacturer change in fluorescence intensity over a 14 day time period. The fluorescence intensities of DNA samples treated with formaldehyde and glutaraldehyde decreased significantly (*p?
