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chitwoodi eggs, the number of eggs at 55 DAI and Pf/Pi ratios revealed that lines D1, D9, D21, D25, D33, and D36 were most resistant against M. chitwoodi. The average number of eggs per plant at 55 DAI in the resistant lines ranged from 263 in D21 to 5,633 in D25, and had corresponding Pf/Pi ratios from 0.22 to 4.69. The reduction was significant (P SNS-032 clinical trial highly resistant line in this screening assay and was chosen for further analysis. Northern blots confirmed a strong expression of 16D10-specific small RNAs in line D21 (Fig. 3). Fig. 1 Southern blot showing copy numbers of the 16D10i-2 RNAi transgene in transformed potato lines and controls. For each plant line, 15 ?g DNA was digested with 50 U Xba I and separated on a 0.8% agarose gel. 82-4, PA99N82-4 advanced breeding line; ... Fig. 2 Number of Meloidogyne chitwoodi ��WAMC1�� eggs per plant in wild type and transgenic RNAi potato lines at 55 days after inoculation. RB, wild type ��Russet Burbank��. Enol Each bar represents the mean of five plants per independent ... Table 1 Reproductive efficiency of Meloidogyne chitwoodi isolate ��WAMC1�� on wild type and 16D10i-2 RNAi transgenic ��Russet Burbank�� potato lines at 55 days after inoculation. Fig. 3 Northern blot for 16D10i-2 RNAi transgene. A. Total RNA loading control. B. U6 small nuclear RNA (snRNA) loading control. C. 16D10i-2-specific FDA-approved Drug Library small RNA (smRNA) using probe 16D10 (Dinh et al., 2014a). 82-4, PA99N82-4 advanced breeding line; RB, wild type ... Broad Meloidogyne resistance in stable transgenic 16D10i-2 potato line D21: When challenged with M. arenaria, M. hapla, M. incognita, or M. javanica, line D21 showed strong resistance regardless of root-knot nematode species. At 35 DAI, the number of egg masses per plant was significantly reduced (P