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The in-vitro studies are mostly designed by using titanium discs however observing the sealing capacity of the varnish at implant abutment junction is more valuable. Aim/Hypothesis The purpose of this study is to evaluate the sealing effect of two varnishes (EC40 and Cervitec) in an oral biofilm in vitro; at the implant abutment junction. Material and methods In the first phase implants and abutments were sealed with EC40 (Sandarac 35%, Chlorhexidine Diacetate 34%, Ethanol 30%) or Cervitec (Chlorhexidine 1%, Thymol 1%) and abutments were screwed onto the implants and torqued Selleckchem GDC0068 20?Ncm with a rachet adaptor. Two different types of implants were used (Tekka, Brignais, France and Biohorizon, Birmingham, AL, USA). A mixed culture of Actinomyces Actinomycetencomitans ATCC 33384, Tannerella Forsythia ATCC 43037, Fusobacterium nucleatum ATCC 25586, Porphyromonas gingivalis, Prevotella intermedia and Streptococcus mutans ATCC 25175 was adjusted to 1?��?108 by McFarland no 0.5 in BHI (Thermo Scientific, UK) broth. Implants sealed with EC4O, Cervitec and control implants were transferred into the mixed culture and incubated anaerobically at 37��C for 72?h. After periods of 7, 14 and 21?days, the sealing capacity and the antimicrobial activity was checked. Statistical analyses Isotretinoin were performed using SPSS version 21 (Chicago, IL). Descriptive statistics were shown with frequency and percentages for categorical data. Chi-Square test was used to compare the frequency distribution of the groups. P��.05 was considered statistically significant. Results Preliminary results showed no bacterial growth after 7 and 14?days with both Tekka and Biohorizon implants sealed with selleck kinase inhibitor EC40. On the 21st day, bacterial counts were observed at 2?��?106 and 32?��?106 respectively in Tekka and Biohorizon implants. Cervitec seal displayed high bacterial counts (123?��?106 and 31?��?106) for Biohorizon implants, on the 7th and 14th days. However, following 21?days the bacterial counts decreased both for Biohorizon and Tekka implants. Despite these changes no significant difference was observed between all groups (P?
