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A P-value content of PCIS from different species was found. Temsirolimus (CCI-779, NSC 683864) When compared with directly after slicing, the ATP content of hPCIS decreased about 30% and 50%, after 48 and 72?h of incubation, respectively (Fig.?(Fig.1A).1A). However, in rPCIS, already after 4?h of incubation the ATP content significantly decreased and after 24?h the ATP content was reduced by 75% compared to freshly prepared PCIS (Fig.?(Fig.1B).1B). In mPCIS, the ATP content was not significantly different after 48?h of incubation, yet, after 72?h of culturing, ATP levels were significantly decreased to 36% as compared to freshly prepared PCIS (Fig.?(Fig.11C). Figure 1 Long-term incubation of hPCIS, rPCIS, and mPCIS. The viability of PCIS as measured by ATP content (relative value compare to 0?h) after incubation of (A) hPCIS up to 72?h, (B) rPCIS up to 24?h, and (C) mPCIS up to 72?h. ... RNA Synthesis inhibitor To evaluate the morphological integrity of PCIS after incubation, the modified Park score was used. An increased Park score indicated a decrease in viability. Mean Park scores of hPCIS increased significantly during 48?h of culture when compared with hPCIS directly after slicing AZD2281 price (Fig.?(Fig.2A).2A). Furthermore, the mean Park score of rPCIS and mPCIS also increased significantly during incubation (Fig.?(Fig.2B2B and C). Very low Park score of nonincubated slices showed that PCIS were not damaged by handling and slicing (Fig.?(Fig.2).2). A significant correlation between ATP content and mean Park scores was found in hPCIS (Spearman r?=??0.76, P?