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2D-flatbed, 2D-MGC and 3D-FHS-MGC images were generated as described for the phantom-resolvability experiment. Acquired 2D-MGC and 3D-FHS-MGC images were compared to 2D-flatbed images. Figure 6 Cluster node resolvability. A. Four 99mTc-pertechnetate sources of 0.5 MBq placed at 10 mm center-to-center distance from each other. B. Four 99mTc-pertechnetate sources of 1 MBq placed at 10 mm center-to-center distance from each other. C. Four 99mTc-pertechnetate ... Patient study Patients Between November 2013 and April 2014 ten patients with Tis-T2 breast cancer scheduled for SN biopsy were included after obtaining written informed consent. All patients were clinically node negative as defined by ultrasound and fine needle aspiration cytology. Further patient characteristics are given in Table 1. The study protocol was Pifithrin-�� manufacturer approved by the institutional review board functioning at the Leiden University Medical Center. Table 1 Patient characteristics and preoperative findings Sentinel node resolvability Patients were injected peri-areolarly with approximately 100 MBq (range 96-108) 99mTc-nanocolloid (0.2 mL; GE Healthcare, Leiderdorp, the Netherlands). Planar RGFP966 research buy anterior, lateral, and oblique lymphoscintigrams (2D-flatbed images; Toshiba) were obtained 15 min and two hours post-injection with an acquisition time of five min. After image acquisition the nuclear medicine physician marked location of the identified SNs on the skin with indelible ink. Injection site-to-SN distances were measured on the late anterior lymphoscintigram using Osirix medical imaging software (Pixmeo, Geneva, Switzerland). Thereafter the marked SN location was placed in the center of the VOI and a 2D-MGC image was made. Then a 3D-FHS-MGC scan was generated and fused with the PDK4 real-time video feed. This allowed hotspot resolvability in augmented-reality and navigation in virtual-reality. Acquired 2D-MGC and 3D-FHS-MGC images were compared to 2D-flatbed images. Results Phantom experiments Detection sensitivity experiment In three setups (Figure 4) a dilution series of 99mTc sources was imaged via conventional 2D-flatbed imaging, 2D-MGC and the 3D-FHS-MGC imaging. While 2D-MGC image acquisition was real-time with an acquisition time of
