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g. French fries, egg, tea and doogh or Iranian yogurt drink). Table 1 Food groups used in the dietary pattern analysis8 Data collection The questionnaires were completed by trained interviewers for cases and controls. This questionnaire included information on socio-demographic characteristics, family history of CRC, physical activity, smoking habit, cooking techniques and dietary intake. Weight was measured to the nearest to 0.1 kg with respondents wearing minimal clothes and without shoes. For patients who had undergone surgery or a long stay in the hospital, the weight at the time of admission was used as current weight. Height was measured using a SECA body meter with a precision of 0.1cm with respondents Ro3280 stand erect, without shoes, their feet together and eyes in a parallax state. For bedridden patients, the recumbent length was obtained in a supine position. Body mass index (BMI) was then calculated as weight (kg) divided by height (meters) squared and was classified according to WHO standard for adults. Physical activity was assessed using a validated self-report questionnaire, Standard metabolic energy equivalent task (MET) times/week, based on reported time spent on different activities which were 3-deazaneplanocin A solubility dmso weighted according to intensity level was applied. Physical activity was completed based on activities during the year before CRC diagnosis (cases) or during the year before interview (controls). Alcohol intake information was not sought from respondents as during the pre-testing of questionnaire, they refused to respond to the item due to cultural and religious beliefs. Iran is an Islamic country where sale and consumption of alcoholic beverages are prohibited. Statistical analysis Data were analyzed with this website SPSS (version 16.0, Chicago, IL, USA). For check of data normality, Kolmogorov-Smirnov test was used. Square root data transformation was applied for data not normally distributed. Dietary patterns were derived using factor analysis by extraction method of Principal Component Analysis (PCA) based on the 123 food items. Prior to extracting factors (patterns), suitability of using factor analysis for this study was assessed using two criteria tests, namely Kaiser-Meyer-Olkin (KMO-test) and Bartlett��s test of Sphericity. Sampling adequacy and inter-correlation of variables were supported by KMO value > 0.647 and Bartlett��s test of Sphericity