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?1a). Phylogenetic analysis of 340?nt of the NS5B region assigned two strains (47015 and 47054) to 1b and all others to genotype 6 (Fig.?1b). One strain each belonged to 6h (47070), 6n (47069), 6k (47098), 6l (clones of 47013) and 6q (47087) and three sequences to 6b (47011, 47026, 47017). The other 30 strains clustered inconclusively (Fig.?1b, Protein Tyrosine Kinase inhibitor unassigned groups 1�C3). Of the three suspected mixed infected samples (47013, 47067 and 47078), only the clones of 47078 clustered differently (with the first and second set of unassigned sequences, Fig.?1b), confirming the mixed infection. There was a good overall agreement between the results obtained for the two different genomic regions with only two sequences showing a distinct clustering (47049, core/E1, 6b; NS5B, unassigned group 1; 47067, core/E1, second cluster of the first unassigned group; NS5B, first cluster of the first unassigned group) (Fig.?1). Genotype 6 of HCV is largely predominant in Laos (43/45, 95.6%), while only two sequences of the worldwide prevalent subtype 1b were found and no subtype 3a, which was suggested to be the most common subtype in south-east Asia [7]. Thus, the genotype prevalence in Laos is quite distinct from that in the surrounding countries, which may be due to less exposure to public health mass interventions [5]. The six genotype 6 subtypes detected in the present study were reported previously mainly from south-east Asia and none of them seems to be restricted to Laos JQ1 solubility dmso [5,7]. Unassigned group 1 seems to represent a distinct cluster of 6j or 6i, due to its clustering in the phylogenetic E-64 trees of Fig.?1 and its minimal genetic distance of