Rare Nevertheless Possible HSP90 Methods

[4] Citrullus colocynthis (colocynth) (C.C) is a member of the Cucurbitaceae superfamily, which is used as a traditional good remedy for the DM.[5] This plant is also known as Hindal in Arabian word[6] and Abu Jahl watermelon in Persian speaking. Due to its bitter flavor, similar to colocynthine, the extract of this fruit was named so. Its ingredients are glycosides (Estrols), alkaloids and flavinoids.[7,8] Estrols (Class A, B, K, L, J and E) are other constituents of C.C.[9,10] The curcurbitacins (highly oxygenated tetracycluic glycosides) are of great interest for therapeutic applications due to their wide board of biological functions exert in plants and animals.[11] These compounds are predominantly found in curcucbitaceae family but are also present in other families of plant kingdom.[11] Another member of this family, Mormordica charantia is a popular traditional HSP90 remedy for DM. C.C is also applicable as purgative, antineoplastic, anti-allergic and anti-rheumatism.[12,13] The dried pulp and seed extracts of C. C are both considered as anti-diabetic agents.[14,15] However, the fruit is mostly used in its intact form traditionally. C.C has been shown to decrease plasma glucose levels in both normo- and hyperglycemic cases.[7] Despite of these known effects, the underlying mechanisms and effective ingredients are not yet clear. The current study aims to investigate the impact of pulp find more and seed of C.C on the hepatic glycogen stores of pharmacologically diabetic rabbits. MATERIALS AND METHODS Extraction The full process was done by a pharmacologist, in Dept. of Pharmacology, Tabriz University of Medical Sciences, Tabriz, Iran. Seeds of dried C.C were separated from pulp, rind and milled mechanically in order to obtain fine particles. Derived powder was then defatted by n-hexan. Extraction process from pulp and defatted seed was done via application of 70% methanol and repeated for three times. The extract was dried at low temperature (2-MeOE2 cost Estrols investigation: Estrols were identified by the creation of blue-green color after addition to anidric acetic plus concentrated sulphoric acid, based on the Libermann-Burchard reaction. Flavonoids investigation: Using Cyanidin test, Flavonoids were detected by production of red or orange color by addition of Hydrochloric acid (HCL) and Mg ribbon in alcoholic milieu. Saponin investigation: Aqueous extracts containing saponin form permanent foam after 2 minutes of vigorous shaking at room temperature. Olive addition in the main solution, leads in emulsion formation.