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Statistical analysis Multiple imputations were performed to account for missing values. Because RVX-208 the imputation method depends on the patterns of the missing data and the types of the imputed variables, without loss of generality, the Markov chain Monte Carlo (MCMC) method provided by the MI procedure of SAS was used.26 Most variables had less than 2% missing observations before imputation, except for smoking and physical activity which had less than 10% missing values. Descriptive statistics were generated for all baseline characteristics according to gender and incident NAFLD or no incident NAFLD. Continuous variables were expressed as the mean and SD (mean��SD), or median (quartile range) based on whether their distribution was normal or skewed as judged by histogram. A two-sample t-test and Wilcoxon rank sum test were used to determine the group difference. Categorical variables were expressed as percentages, and ��2 tests were used to determine the difference for categorical data. Additionally, WBC (cells 109/L) were further categorised separately into quartiles: Q1: WBC? BMS-777607 purchase hypertension, hyperglycaemia, TC, TG, HDL-C, LDL-C, ALB and GLO at baseline. SAS V.9.1.3 (SAS Institute, Cary, North Carolina, USA) and SPSS (V.20.0) were used to perform all statistical analyses. Results A total of 15?201 subjects (7286 men and 7915 women) were included in this study. The baseline distributions of age, WBC, BMI, SBP, DBP, FPG, TC, TG, HDL-C, LDL-C, TP, ALB and GLO by gender; the prevalence of hypertension, hyperglycaemia, smoking and regular exercise; and comparisons between males and females are summarised in table 1. The mean age Neratinib solubility dmso was 45.49��16.49?years in males and 40.60��13.00?years in females. All other variables except TC were significantly different between males and females (p