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About 2 cc of blood were collected using a Vacuum Serum Separation Tube (SST; Green Cross Corp., Yongin, Gyeonggi, Republic of Korea) and left at room temperature for 1 hour. Serum was isolated from the blood samples by centrifugation at 1977 g and 4��C for 20 minutes, then stored at ?20��C. A Hitachi Clinical Analyzer 7080 (Hitachi Korea Ltd., Seoul, Republic of Korea) was used to measure the serum concentrations of various lipid components including TG, T-CHO, HDL cholesterol, and LDL cholesterol. Histological analysis by Oil Red O staining At 24 hours after the final oral administration of the experimental diets, liver tissues were harvested from the sacrificed rats and immediately frozen at ?80��C. Frozen liver tissues were cryo-sectioned (6 ��m thick), fixed in a 10% formalin solution (OCI selleck compound Company Ltd.) at 4��C for 5 minutes, and then rinsed three times with distilled water. A 5% Oil Red O working solution was prepared by dissolving Oil Red O powder (Sigma-Aldrich) in propylene glycol (OCI Company Ltd.), and then used to stain the sectioned tissues according selleck kinase inhibitor to the manufacturer's instructions. Counter-staining was conducted with hematoxylin (Sigma-Aldrich), and the sections were then mounted in glycerine (OCI Company Ltd.). Lipid-containing cells were detected as those containing red inclusions using a light microscope (BX51 U-LH100HGWIG, Olympus, Tokyo, Japan; 100�� and 200�� magnification). Statistical analysis All data were analyzed using GraphPad Prism software (San Diego, CA, USA). In vitro data are presented as the means �� SEM. A one-way ANOVA was conducted followed by Dunnett's multiple comparison test. P-values Fleroxacin to n-3 oils ratio (n-6:n-3) of 0.08 (Table 2). Comparison of the amount of PUFAs revealed that microalgal oil contained more PUFAs than the commercial omega-3 fish oil and that this commercial fish oil contained much more saturated fatty acids than PUFAs, contrary to expectations from its label as omega-3 oil. The commercial fish oil was not found to be authentic omega-3 oil because it only contained omega-3 oils in part. Table 1 Fatty acid composition of microalgal oil derived from Thraustochytriidae sp. derived mutant Table 2 Fatty acid composition of commercial omega-3 fish oil Body weight The average body weight of mice at the beginning of the experiment was (21.2 �� 0.94) g, and this increased to (31.18 �� 1.
