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Samples have been ready for Western blot analysis in the existence of a protease inhibitor cocktail, which inhibits the activity of the proteasome, calpains and caspases but not that of lysosomal enzymes	Samples shown to be b-globin positive were then examined for bacterial quantification by Real time PCR	Samples were being ready for Western blot evaluation in the existence of a protease inhibitor cocktail, which inhibits the exercise of the proteasome, calpains and caspases but not that of lysosomal enzymes
Samples were homogenized and a portion of the supernatant was reserved for protein determination (BCA Assay, Pierce)	Samples were subjected to immunoblotting using a monoclonal GST, a monoclonal PEDF and monoclonal Transportin-SR2 antibody	Sampling site and time point of infection, considering that Tregs are inversely
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