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8485. Oral glucose tolerance test[17,18] Oral glucose selleckchem tolerance test (5 g/kg BW) was performed on normoglycemic mice (n = 9), followed by measurement of blood glucose level (mg/dl) using OneTouch Horizon (Lifescan, Johnson and Johnson, USA). Polysorbate 80 (25 ml/kg BW, UNIVAR, Australia) and distilled water were used as negative controls; Solosa (1.67 ��g/kg BW, Glimepiride solosa, Aventis, Italy) dissolved in distilled H2O was used as positive control; and crude aqueous leaf extract (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW and 25 mg/kg BW) dissolved in distilled water, DCM leaf extract (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW and 25 mg/kg BW) dissolved in Polysorbate 80 (25 mg/kg BW) and 1 (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW, and 25 mg/kg BW) dissolved in polysorbate 80 (25 ml/kg BW) as test samples were orally administered to male albino mice. Blood glucose was Akt cancer measured within a 3 h period at 30 min intervals. Blood glucose reduction was computed and was used in the statistical analysis. Statistical analysis The results were analyzed using GraphPad Prism Version 6a (GraphPad Software Inc., La Jolla, CA, USA). One-way analysis of variance was performed to determine the significant effects of the hypoglycemic potentials of 1 and the aqueous and DCM extracts of G. oppositifolis leaves. The results were considered significant at P Montelukast Sodium test. Values are presented as mean �� standard deviation (SD). Analgesic effect The tail flick assay[19,20] was conducted on mice (n = 7) orally administered with Diclofenac (7.14 mg/kg BW) (GX International, Munti + nlupa City Philippines) and Polysorbate 80 (0.025 ml/g BW) as the positive and negative controls, respectively, and three dosages (50, 100 and 200 mg/kg BW) of aqueous leaf extract and DCM leaf extract and 1 dissolved in polysorbate 80. One-third of the distal part of the tail was immersed in a warm water bath (50��C) 1 h after the treatments. The time the mouse withdrew its tail from the water bath was noted. Percent inhibition was calculated according to the equation: % Analgesic effect = 100�C([time that the experimental mice attempted to remove their tails/average time that the control mice attempted to remove their tails] 100). Antimicrobial test Test bacterial isolates The test bacterial isolates were taken from the DLSU Biology Department's microbial culture stocks that are maintained at ?80��C. The purity of the cultures was ascertained, and the resistance phenotypes were confirmed using the standard disc diffusion method of Clinical and Laboratory Standards Institute (2010).