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One-way analysis of variance was used for between-group comparisons of numerical variables. Post hoc analysis was performed using Tukey's test for between-group comparisons of categorical variables. Student's t-test and the Mann�CWhitney U-test were also used for analysis of difference of means for the quantitative Megestrol Acetate data. P All the three groups were homogeneous with respect to age, sex, body weight and duration of surgeries [Table 1]. Table 1 Demographic profile The CB was adequate in 85 out of 90 cases and did not require any further supplementation of analgesics intra-operatively for the surgery to proceed. The anal sphincter was relaxed in 89.3%, 82.1% and 75% of the cases in groups BD0, BD1 and BD2, respectively, 5 min after the administration of the CB. This difference between the three groups was not statistically significant (P = 0.378). At the end of surgery, however, the anal sphincter was lax in all the patients. There were no significant differences in the heart rates and mean blood pressures within the groups over time or between the groups at any time interval [Figures ?[Figures22 and ?and33]. Figure 2 Changes in heart rate (mean��SD) Figure 3 Changes in mean arterial pressure(mean��SD) The time to first analgesic requirement (total duration of post-operative analgesia) in the BD0 group was 444.6 �� 179.4 min (range of 4�C13 h); whereas in the BD1 group, it was 964.2 �� 39 min (range of 4�C24 h) and in the selleck chemicals BD2 group, it was 1152.6 click here �� 380.4 min (range of 4�C24 h) [Table 2]. The differences in the mean duration of analgesia were highly significant (P