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Generalized estimating equations were used to estimate odds ratios (OR) for the association between lifestyle groups and sensitization, which was measured at 6, 12, and 24?months of age. Median regression was used for total IgE because it showed a skewed distribution. To adjust for the dependence of total IgE within each child, cluster bootstrap was used to estimate standard errors. Data were adjusted for the following baseline variables: sex of the child, AG-221 mouse parental sensitization, maternal smoking during pregnancy, number of siblings or other children living with the family, exclusive breastfeeding at 6?months of age, family living on a farm with animals during pregnancy, and parental education. A P-value RSL3 concentration not continue in the study for various reasons: nine because of stressful situations, two because of disease other than allergy of the child or mother, three because of moving, and three for unknown reasons. Five families did not answer the questions used for categorization into lifestyle groups and were therefore excluded from this report. Thus, after categorization, the study included 82 families with anthroposophic, 120 with partly anthroposophic, and 100 with nonanthroposophic lifestyle. After 2?months of age, an additional five families left the study until the age of 24?months: two because of disease other than allergy of the child, one because of moving, and two for unknown reasons. Demographic and allergy-related data in the families are shown in Table?2. In all lifestyle groups, the majority of parents were born in Scandinavia. However, in the anthroposophic group, parents to a higher extent were born abroad, mostly Succimer in another European country. The education level of the parents was similar between the three groups. Families with nonanthroposophic lifestyle more often lived in private houses as compared with the other groups. Parental allergic sensitization and reported symptoms of allergy-related disease in the families did not differ significantly between the three lifestyle groups. In total, 27.2% of the mothers were IgE-sensitized and 43.0% of the fathers. However, many lifestyle factors differed markedly between the groups during pregnancy, delivery, and the first 2?months of age of the children (Tables?3 and 4), for example concerning diet, place of delivery, and medication.