Thus, 2D2 T cells possess a relatively higher functional avidity for NFM, with proliferation and IL-2 production closer towards the corresponding functional avidity of SMARTA cells than to 2D2 cells for MOG

Wanda Von Kruger for use of electroporation facilities and Dr. Marta T. Gomes for support with all the lipid analyses. We are indebted to Jose Nilson dos Santos, Jose F. Tiburcio and Talita M. Oliveira for technical help. Author Contributions Conceived and created the experiments: LF-M AHL RL. Performed the experiments: LF-M TM RMM RQM ME-L RRB. Analyzed the information: LF-M TM RMM RQM ME-L RRB AHL RL. Contributed reagents/ materials/analysis tools: LF-M AHL RL. Wrote the paper: LF-M AHL RL. like lipid within the developing retina. A: Thin layer chromatography of commercial PAF, and of lipids in the neonatal retina, extracted in either ethanol or chloroform. Corresponding spots of commercial PAF and retinal PAF-like 12 January 2011 | Volume 6 | Challenge 1 | e16058 PAF-Induced Arrest of Retinal Cell Cycle 20. Pearson RA, Luneborg NL, Becker DL, Mobbs P Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells. J Neurosci 25: 1080310814. 21. Liu X, Hashimoto-Torii K, Torii M, Ding C, Rakic P Gap junctions/ hemichannels modulate interkinetic nuclear migration in the forebrain precursors. J Neurosci 30: 41974209. 22. Tsai LH, Gleeson JG Nucleokinesis in The outcomes indicate that miR-302b is definitely an essential miRNA related for the inhibition of cancer progenitor cells caused by Ascl2 selective blockade in HT-29 cells neuronal migration. Neuron 46: 383388. 23. Morris NR, Efimov VP, Xiang X Nuclear migration, nucleokinesis and lissencephaly. Trends Cell Biol 8: 467470. 24. Wynshaw-Boris A, Gambello MJ LIS1 and dynein motor function in neuronal migration and improvement. Genes Dev 15: 639651. 25. Morris NR Nuclear migration. From fungi to the mammalian brain. J Cell Biol 148: 10971101. 26. Gambello MJ, Darling DL, Yingling J, Tanaka T, Gleeson JG, et al. Multiple dose-dependent effects of Lis1 on cerebral cortical development. J Neurosci 23: 17191729. 27. Arai H, Koizumi H, Aoki J, Inoue K Platelet-activating aspect acetylhydrolase. J Biochem 131: 635640. 28. Stafforini DM, McIntyre TM, Zimmerman GA, Prescott SM Plateletactivating aspect, a pleiotrophic mediator of physiological and pathological processes. Crit Rev Clin Lab Sci 40: 643672. 29. Ishii S, Shimizu T Platelet-activating aspect receptor and genetically engineered PAF receptor mutant mice. Prog Lipid Res 39: 4182. 30. Shukla SD Tyrosine kinase activation by PAF results in downstream gene expression. Adv Exp Med Biol 416: 153155. 31. Albrecht U, Abu-Issa R, Ratz B, Hattori M, Aoki J, et al. Plateletactivating factor acetylhydrolase expression and activity recommend a hyperlink in between neuronal migration and platelet-activating aspect. Dev Biol 180: 579593. 32. Hirotsune S, Fleck MW, Gambello MJ, Bix GJ, Chen A, et al. Graded reduction of Pafah1b1 activity leads to neuronal migration defects and early embryonic lethality. Nat Genet 19: 333339. 33. Bix GJ, Clark GD Platelet-activating factor receptor stimulation disrupts neuronal migration in vitro. J Neurosci 18: 307318. 34. Tokuoka SM, Ishii S, Kawamura N, Satoh M, Shimada A, et al. Involvement of platelet-activating aspect and LIS1 in neuronal migration. Eur J Neurosci 18: 563570. 35. de Araujo EG, Linden R Trophic variables made by retinal cells improve the survival of retinal ganglion cells in vitro. Eur J Neurosci 5: 11811188. 36. Alexiades MR, Cepko C Quantitative evaluation of proliferation and cell cycle length through development on the rat retina. Dev Dyn 205: 293307. 37. Dover R, Patel K Enhanced methodology for detecting bromodeoxyuridine in cultured cells and tissue sections by immunocytochemistry. Histochemistry 102: 383387. 38. Campos