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The number of GFP-positive Megestrol Acetate surviving SGNs in NT-3 across all repetitions was 127 �� 24 (mean �� SD). Similar to wild-type GFP-Bcl-2 (Hansen et al., 2007), both GFP-Bcl-2-Maob and GFP-Bcl-2-Cb5 promote SGN survival compared with GFP-Maob or GFP-Cb5, respectively (P Birinapant in vitro et al., 2001). Thus, similar to wild-type Bcl-2 and Bcl-XL, Bcl-2 targeted to learn more the mitochondria or ER inhibits SGN neurite growth. To characterize further the prosurvival effects of Bcl-2, we asked whether the ability of Bcl-2 targeted to either the mitochondria or the ER to promote SGN survival was additive with neurotrophins. SG cultures transfected with GFP-Bcl-2-Maob, GFP-Bcl2-Cb5, GFP-Maob, or GFP-Cb5 were maintained in the presence of absence of NT-3 (50 ng/ml), and SGN survival was determined as described above. There was no difference in SGN survival in cultures transfected with GFP-Bcl-2-Cb5 in the presence of absence of NT-3, indicating that the prosurvival effects of neurotrophins are not additive with Bcl-2 localized to the ER (Figs. 3, 5). By contrast, treatment with NT-3 significantly reduced the survival of SGNs transfected with GFP-Bcl-2-Moab relative to those maintained in the absence of NT-3 or to SGNs transfected with GFP-Maob and maintained in NT-3 (P