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It can be seen that cells from MVA��C12L/��A44L-A46R immunized mice showed significant higher cytoxicity capacity Cabozantinib compared to cells from MVAwt immunized mice (p mice showed a higher functionality compare to those from MVAwt. Figure 7 Study of in vitro T-cell specific killing activity. Specific cytotoxicity assay against B8R-pulsed cells was performed as described in Materials and Methods. Data are expressed as mean + SD of triplicate determinations and represent two independent experiments. ... 3.6. Pattern of Cytokines Induced during Innate Immune Response by MVA?C12L/?A44L-A46R The experiments described above showed tuclazepam that MVA?C12L/?A44L-A46R induced adaptive T-cell responses of higher magnitude and quality vs. MVAwt. Consequently, we analyzed the cytokine pattern of the innate immune response elicited in vivo by this vector compared to MVAwt. Groups of four to six C57Bl/6 mice immunized with MVAwt or MVA?C12L/?A44L-A46R were sacrificed at different time points (4�C6, 16�C20 and 30 hpi), and diverse pro-inflammatory mediators were evaluated in homogenates of ILNs (Figure 8a) and serum (Figure 8b). In ILNs animals inoculated with MVA?C12L/?A44L-A46R showed an increase in the levels of IFN-�� (p = 0.054), and importantly significant higher levels of IL-12 (p 8a) compared to MVAwt. We also Tofacitinib molecular weight analyzed the percentages of mice whose responses overcame the na?ve median for each cytokine and the fold induction in each group, in relation to the na?ve group. As observed, in the tables depicted below of each cytokine-graph, except for the shorter time-point for IL-12 and IL-1��, the MVA?C12L/?A44L-A46R induced higher percentages of mice with responses over the na?ve value and also superior fold-inductions vs. MVAwt vector for all cases. Figure 8 Analysis of the innate immune response in vivo. C57Bl/6 mice were i.m. immunized with 2 �� 107 PFU of MVAwt (black) or MVA?C12/?A44L-A46R (white) and at indicated hours post-inoculation (hpi) were sacrificed and samples processed ... In sera we only found detectable values for IFN-��, being higher in mice inoculated with MVA?C12L/?A44L-A46R vs. MVAwt at 4�C6 hpi (p