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The C2 and D1 responses were normalized to the maximum C2 and D1 responses, respectively. The relationship between the normalized responses and C1 durations was analyzed using linear regression analysis (significance level, P?BIBF 1120 molecular weight Mean firing rates during the D2 period were calculated separately for different C2 durations in LS and SL trials. Using linear regression analysis (significance level, P?GS-7340 order C2 and red C2 trials in C2 response neurons. When a statistically significant difference was detected in one of these comparisons, the neuron was defined as a color-sensitive neuron. In D2 response neurons, D2 activity was compared between blue-choice and red-choice trials using the Mann�CWhitney U-test (significance level, P?Adenylyl cyclase negative currents (30?��A delivered for 30?sec) through the microelectrode in the striatum. The monkey was deeply anesthetized with an overdose of sodium pentobarbital (Nembutal; 60?mg/kg, i.p.), and perfused transcardially with 0.1?mol/L phosphate-buffered saline (PBS, pH 7.3), followed by 10% formalin dissolved in 0.1?mol/L PBS. The brain was removed immediately from the skull, saturated with 10% then 30% sucrose in 0.1?mol/L PBS at 4��C. The saturated brain was cut serially into 50?��m thick coronal sections on a freezing microtome. Nissl staining was performed in every fourth section to trace the penetrations and verify the electrolytic lesions. Recording sites were reconstructed by reference to marking lesions, and aided by the log notation of the micrometer depths at which different types of neuronal activity were recorded as the electrode was advanced.