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Serum insulin (?U/mL) (LINCO Research, St Charles, MO, USA) will be measured using enzyme-linked immunosorbent assay (ELISA), and serum glucose (mg/dL) (Stanbio Labs, Boerne, TX, USA) will be measured by spectrophotometry. Total cholesterol (mg/dL), HDL-C (mg/dL) and TG (mg/dL) concentrations will be measured by spectrophotometry using the total cholesterol, HDL-C and TG kits, respectively (Stanbio Labs). Total cholesterol, HDL-C and TG concentrations will be used to calculate LDL-C concentration (mg/dL) using the equation: LDL-C=total cholesterol - HDL-C - (TG/5) [42]. Serum leptin (ng/mL), adiponectin (ng/mL), and resistin (ng/mL) will be measured using ELISA (R&D Systems, Minneapolis, MN, USA). All serum samples for each biomarker will be analyzed in duplicate at corresponding study Histone demethylase intervals. Intra- and inter-assay coefficients of variations (CV) are Enzalutamide nmr health perceptions, perseverance, and SCT mediators. The Eating Inventory [43] will evaluate ratings of cognitive eating restraint, hunger, and disinhibition. The Short-Form 36 Health Survey (SF-36) [44] will assess self-reported health issues. Perseverance will be examined using the Short Grit Scale (Grit-S) [45], and an investigator-designed questionnaire will evaluate SCT mediators, including self-efficacy, outcome expectations, self-regulation, and social and family support. Standard scoring and interpretation methods will be used to evaluate all questionnaires [43-45]. Statistical Analysis Baseline characteristics of study participants were characterized using descriptive statistics: mean (SD). Participants in the three intervention groups (Treatment) will participate in five data collection sessions at specified intervals (Time). The Shapiro-Wilk test for normality will be used to test for normality and homogeneity of variance within groups; data will be transformed if necessary. Body weight, BMI, waist : hip Venetoclax datasheet ratio, FM, BF%, systolic and diastolic blood pressure, serum insulin, glucose, TC, HDL-C, LDL-C, TG, leptin, adiponectin, and resistin will be analyzed as dependent variables. Baseline variables that differ between groups will be included as covariates in the analysis. Dietary intake of macronutrients as estimated from 4-day food records, estimated energy expenditures, eating behaviors, health perceptions, and ratings of SCT mediators also will be compared among groups. A 3 x 5 (3 treatment groups x 5 time intervals) ANOVA with repeated measures on the time factor will be used to assess differences in outcomes within and between treatment groups over time.

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