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In negative mode, more m/z peaks were present in a mid mass range (m/z 401�C600) however in a higher mass range (m/z 801�C1000) a similar number of peaks were detected in positive and negative modes. Absence of salt adducts in negative mode allowed better detection of molecular species in a wide mass range (92 m/z peaks in negative mode vs. 79 m/z peaks in positive, respectively) due to an enhanced signal-to-noise ratio and to a difference in the heterogeneity of the sample modifying the ion suppression Selleck Lapatinib effect. We did quantitative analyses of the spectra extracted from either FF or OCC, or GC or theca in the representative follicles, for both positive and negative ion mode datasets to confirm the visual differences in spatial distribution of lipid species between the follicular compartments. For each m/z peak, the variations in signal intensities between each compartment were compared (Supplemental data, S1). Peaks at 35 and http://www.selleckchem.com/products/LY294002.html 59 m/z, detected in positive and negative ion mode, respectively, varied between at least two different compartments (p S6 Kinase 3-fold higher than in OCC. Figure 6 Comparative analysis of the 25 lipid species that differed most among follicular compartments detected by MALDI MSI in positive and negative ion mode. Log-values of normalized peak heights of the ions detected in follicular fluid, theca, granulosa and ... This differential analysis based on quantitative MSI profiles corroborates the images of ion density maps of differential m/z species (Figure 7). The lipid species recorded in negative ion mode at 539.9 and 859.5 m/z showed variability in intrafollicular distribution, with a specific localization in FF and GC, respectively. The intensity of the lipid ion observed in positive ion mode at 820.4 m/z was two times greater inside OCC than in other tissues, most likely corresponding to the oocyte (Figure 7, arrow pointing at OCC).