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The methods were Fluorescence Polarization Immunoassay (FPIA) for CsA and monoclonal antibody microparticle enzyme immunoassay for TAC [23]. Freeman et al. observed that concentrations of TAC were stable at room temperature, +4��C, and ?70��C for the two-week study period in their E-64 study using the microparticle enzyme immunoassay method. They also reported that the samples stored at ?70��C for almost one year and then reanalyzed were found to be stable [24]. In our study, we observed that the stability of TAC did not change over a two-day period for samples stored at +4��C or a one month period at ?20��C (Table 1). This was consistent with previous studies. However, concentrations of TAC were decreased in samples that were left out at room temperature. CsA levels were stable at ?20��C over a one month period. They were unstable when they were kept at room temperature or +4��C (Table 1). This was not consistent with previous studies. This incompatibility may be caused by the method that we used is different. We used LC-MS/MS in our study because it is a rapid, specific, sensitive, and accurate reference method [25]. Studies showed that LC-MS/MS had significant reproducibility and accuracy advantages compared to Fluorescence Polarization Immunoassay, microparticle enzyme immunoassay, and conventional HPLC-UV methods for the quantitation of TAC and CsA concentrations in whole blood [26, 27]. Based on these results, to accurately determine the level of CsA samples should be measured immediately or R428 stored at ?20��C until analysis. In conclusion, the stability of TAC and CsA depends on storage conditions, which must be considered for accurate measurement. Furthermore, these findings can give http://www.selleckchem.com/products/jq1.html an idea about the conditions under which the serum samples should be kept for TAC and CsA measurement. Further studies with larger samples are needed to delineate this relationship. Acknowledgment The authors are grateful to Dicle University DUBAP for their sponsorship of English editing of this paper. Disclaimer The authors alone are responsible for the content and writing of the paper. Conflict of Interests The authors report no conflict of interests.""Carmine is a natural red pigment extracted from dried scale insects (cochineal, Dactylopius coccus) [1]. The main chemical component of this pigment is carminic acid (7-��-d-glucopyranosyl-9,10-dihydro-3,5,6,8-tetrahydroxy-1-methyl-9,10-dioxoanthracene carboxylic acid, Figure 1). Carmine is widely used for coloring food products, cosmetics, and medicines [2, 3]. In 2012, the Japanese Consumer Affairs Agency published a report on carmine detailing its potential links to dyspnea and anaphylactic reactions [4]. This report detailed approximately 20 articles from 1960s about anaphylaxis resulting from the use of the cosmetics or consumption of food containing carmine.