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The blood samples were obtained from the animal��s heart amounted to 0.5�C2 ml by syringe. Soon after the sampling, the blood was maintained in glasses containing the anticoagulant, EDTA (Ethylene-Diamine-Tetra-Acetic acid), and the leukocytes were counted using the diluent solution, Marcanu (RBC lysis buffer) and the Neobar slide (hemocytometer) by using the light microscope (Olympus, 3H-Z-Japan), In order to count and determine the leukocyte subgroups (including neutrophils, lymphocytes, monocytes and eosinophils) the appropriate peripheral blood smears were prepared on the microscope slides and then fixed by means of the water-free methanol. Then Giemsa staining (Merck-Germany) was carried out by the diluted stain with the rate of 1/10. And finally, the differential counting was performed using a 100x lens microscope (Mahbod 2008, Mansouri et al. 2015). Statistical SCR7 Analysis Data were analyzed using the SPSS ver. 13 (version 13, SPSS Inc, Chicago, IL) and the statistical tests of ANOVA and LSD. Data were considered significant statistically when PMianserin HCl (�� 10 per ml3) in peripheral blood of rat between the groups receiving venom with the concentration of 0.01 and 0.1 mg/kg and the control group at different times Fig. 5. The comparison of the Mean��SD of eosino-phil (�� 10 per ml3) in peripheral blood of rat between the groups receiving venom with the concentration of 0.01 and 0.1 mg/kg and the control group at Entinostat different times Fig. 3. The comparison of the Mean��SD of lymphocytes (�� 10 per ml3) in peripheral blood of rat between the groups receiving venom with the concentration of 0.01 and 0.1 mg/kg, and the control group at different times Results The Effect of H. lepturus Venom on Leukocyte Count The scorpion venom has led to a significant reduction of leukocytes in the venom-receiving group with the dose of 0.01 mg/kg at two and 24 hours after the injection, compared with the control group (P 0.05). The toxicity effect of the venom is intensified with the increase of the injected venom concentration, so that in spite of a decrease in venom effect in the group receiving 0.01 mg/kg after 48 hours, the decrease in toxicity effect is still significant in the group receiving 0.1 mg/kg compared with the control group (P