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34 Time domain measures of HRV were calculated from the RR interval tachograms. The following time domain measures of HRV were calculated: mean RR (NN mean) in milliseconds, the standard deviation of RR intervals in milliseconds and the root mean square successive difference of RR intervals (rMSSD) in see more milliseconds. The following standard frequency domain measures of HRV were computed: high-frequency spectral power (HF power, the density of the beat-to-beat oscillation in the N-N interval of HRV in the high-frequency band; HF?=?0.15�C0.4?Hz), low-frequency spectral power (LF power, the density of the beat-to-beat oscillation in the N-N interval of HRV in the low-frequency band; LF?=?0.04�C0.15?Hz), the ratio of LF to HF power (LF/HF ratio). The LF/HF ratio has been Dipivefrine described as a marker of sympathetic to parasympathetic balance.30,35,36 Spectral components were expressed both as absolute values in milliseconds and as normalized units (nu). To reflect the degree of parasympathetic modulation of heart rate we used both HF power (%) and rMSSD (ms).30�C33 3?mL of venous blood was collected to measure CRP using nephelometry (T60, Siemens AG, Munich, Germany). B-natriuretic peptide was measured by fluorescence immunoassay (T60, Siemens AG, M��nchen, Germany). CRP was measured by immunoluminometry and nephelometry. A statistical software package was used for all calculations (SPSS for Windows, version 11.0, SPSS Inc., Chicago, IL, USA). The physiotherapist conducting the 6MWD and the technician conducting the spirometry were not involved in data analysis. Descriptive data for continuous variables are expressed as mean?��?SD. Univariate (Pearson's correlation) and multivariate analyses were performed to assess the associations between HRQL and anthropometric characteristics (BMI), pulmonary function parameter (FEV1, RV/TLC, DLCO), cardiac autonomic modulation (rMSSD), respiratory symptoms (dyspnoea, 0�C10 points), cardiopulmonary exercise capacity (6MWD) and systemic inflammation (CRP). CX-5461 manufacturer Variables significantly associated with HRV in the univariate analysis were included in a multivariate analysis to assess the independence of the association between rMSSD and HRQL. A P-value of